蒙古马高负荷运动训练前后转录组差异表达分析

doi:10.11843/j.issn.0366-6964.2017.06.005

Abstract

Abstract:

The aim of this study was to analyze the Mongolian horse transcriptome differential expression before and after a high load exercise training. This research selected 6 Mongolian horses with high load training for 4 months. Muscle samples were collected before and after the training respectively. The transcriptome library was established using the second generation sequencing technology. We enriched the differentially expressed genes to the GO and KEGG pathway, and screened the significantly different metabolic pathways and related genes. 1 102 differentially expressed genes(DEGs) in transcriptome library were screened out, of which 299 were down-regulated and 803 were up-regulated after high load training, respectively. These DEGs were annotated to 3 398 GO terms, including muscle structure development, cardiovascular system development, circulatory system development, muscle tissue development, muscular organ development, muscle contraction, muscle cell differentiation, etc. Also the DEGs were annotated to 193 KEGG pathways including some athletics related pathways: dilated cardiomyopathy (DCM), hypertrophic cardiomyopathy, cardiac muscle contraction, calcium signaling, the actin cytoskeleton adjustment, etc. Our findings provide a theory support for further investigating the molecular mechanism of Mongolian horse strong stamina and a new research method for Mongolian horses athletic characteristics.

CLC Number:

S821.2

ZHAO Qi-nan, MANG Lai, BAI Dong-yi, ZHAO Yi-ping, LI Bei, Unerhu, WU Ying-ga, SU Ri-ga, AO Ru-ga. Mongolian Horses Transcriptome Differential Expression Analysis before and after a High Load Exercise Training[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1007-1016.

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References

[1] 吴克亮, 吴常信. 马科学研究动态和马业发展[J]. 畜牧兽医学报, 2005, 36(4):412-416.
WU K L, WU C X. Advanced research on equine science and horse industry[J]. Acta Veterinaria et Zootechnica Sinica, 2005, 36(4):412-416. (in Chinese)
[2] 芒来. 蒙古马的历史与展望[J]. 马业杂志, 2002(4):14-17.
MANG L. History and outlook of the Mongolian horse[J]. Journal of Horse Industry, 2002(4):14-17. (in Chinese)
[3] MOSHER D S, QUIGNON P, BUSTAMANTE C D, et al. A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs[J]. PLoS Genet, 2007, 3(5):e79.
[4] ZHANG X M, ZHAO L, LARSON-RABIN Z, et al. De novo sequencing and characterization of the floral transcriptome of Dendrocalamus latiflorus (Poaceae:Bambusoideae)[J]. PLoS One, 2012, 7(8):e42082.
[5] VARSHNEY R K, SIGMUND R, BÖRNER A, et al. Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice[J]. Plant Sci, 2005, 168(1):195-202.
[6] CÁNOVAS A, RINCON G, ISLAS-TREJO A, et al. SNP discovery in the bovine milk transcriptome using RNA-Seq technology[J]. Mamm Genome, 2010, 21(11-12):592-598.
[7] LI X Y, SUN H Y, PEI J B, et al. De novo sequencing and comparative analysis of the blueberry transcriptome to discover putative genes related to antioxidants[J]. Gene, 2012, 511(1):54-61.
[8] GOECKS J, NEKRUTENKO A, TAYLOR J, et al. GALAXY:a comprehensive approach for supporting accessible, reproducible, and transparent computational research in the life sciences[J]. Genome Biol, 2010, 11(8):R86.
[9] JAILLON O, AURY J M, NOEL B, et al. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla[J]. Nature, 2007, 449(7161):463-467.
[10] CHEN Q M, WANG H, ZENG Y Q, et al. Developmental changes and effect on intramuscular fat content of H-FABP and A-FABP mRNA expression in pigs[J]. J Appl Genet, 2013, 54(1):119-123.
[11] BONNET A, BEVILACQUA C, BENNE F, et al. Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection[J]. BMC Genomics, 2011, 12:417.
[12] MILLER J R, KOREN S, SUTTON G. Assembly algorithms for next generation sequencing data[J]. Genomics, 2010, 95(6):315-327.
[13] http://asia.ensembl.org/Equus_caballus/Info/Index.
[14] ERLICH Y, MITRA P P, DELABASTIDE M, et al. Alta-cyclic:a self-optimizing base caller for next-generation sequencing[J]. Nat Methods, 2008, 5(8):679-682.
[15] ESTEVE-CODINA A, KOFLER R, PALMIERI N, et al. Exploring the gonad transcriptome of two extreme male pigs with RNA-seq[J]. BMC Genomics, 2011, 12:552.
[16] KANG X L, LIU G, LIU Y F, et al. Transcriptome profile at different physiological stages reveals potential mode for curly fleece in Chinese Tan sheep[J]. PLoS One, 2013, 8(8):e71763.
[17] LOBJOIS V, LIAUBET L, SANCRISTOBAL M, et al. A muscle transcriptome analysis identifies positional candidate genes for a complex trait in pig[J]. Anim Genet, 2008, 39(2):147-162.
[18] ANDERS S, HUBER W. Differential expression analysis for sequence count data[J]. Genome Biol, 2010, 11(10):R106.
[19] NIELSEN M, HANSEN J H, HEDEGAARD J, et al. microRNA identity and abundance in porcine skeletal muscles determined by deep sequencing[J]. Anim Genet, 2010, 41(2):159-168.
[20] KEGG:Kyoto encyclopedia of genes and genomes[EB/OL]. http://www.genome.jp/kegg/.
[21] DUTTA S, KUMAWAT G, SINGH B P, et al. Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea[Cajanus cajan (L.) Millspaugh] [J]. BMC Plant Biol, 2011, 11:17.
[22] ZORZATO F, FUJII J, OTSU K, et al. Molecular cloning of cDNA encoding human and rabbit forms of the Ca²⁺ release channel (ryanodine receptor) of skeletal muscle sarcoplasmic reticulum[J]. J Biol Chem, 1990, 265(4):2244-2256.
[23] GAJDOSIK R L. Passive extensibility of skeletal muscle:review of the literature with clinical implications[J]. Clin Biomech, 2001, 16(2):87-101.
[24] SCHRÖDER W, KLOSTERMANN A, DISTL O. Candidate genes for physical performance in the horse[J]. Vet J, 2011, 190(1):39-48.
[25] MANICKAM R, PENA R N, WHITELAW C B A. Mammary gland differentiation inversely correlates with GDF-8 expression[J]. Mol Reprod Dev, 2008, 75(12):1783-1788.
[26] BRYANT N J, GOVERS R, JAMES D E. Regulated transported transport of the glucose transporter GLUT4[J]. Nat Rev Mol Cell Biol, 2002, 3(4):267-277.
[27] 张万坡, 田文霞, 李建丽, 等. 禽流感病毒NS1蛋白与鸡LY6E蛋白间的相互作用[J]. 中国兽医学报, 2008, 28(6):627-630, 645.
ZHANG W P, TIAN W X, LI J L, et al. Interaction between nonstructural (NS1) protein of avian influenza virus and LY6 E proteins of chicken[J]. Chinese Journal of Veterinary Science, 2008, 28(6):627-630, 645. (in Chinese)
[28] 吴帅, 简斌. BCL9与肿瘤及肿瘤靶点治疗研究[J]. 生命科学研究, 2013, 17(1):86-89.
WU S, JIAN B. Research on BCL9 with tumor and tumor target treatment[J]. Life Science Research, 2013, 17(1):86-89. (in Chinese)
[29] 邹剑, 周后凤, 先志伟, 等. 激活PPARα表达对AngⅡ诱导的心肌细胞肥大及NFATc4与p65-NFκB相互作用的影响[J]. 中国病理生理杂志, 2014, 30(6):1017-1022.
ZOU J, ZHOU H F, XIAN Z W, et al. Effects of PPARα activation on AngⅡ-induced cardiomyocyte hypertro-phy and interaction of NFATc4 with p65-NFκB[J]. Chinese Journal of Pathophysiology, 2014, 30(6):1017-1022. (in Chinese)
[30] 王永珍. 峨眉树蛙和斑腿树蛙MHCⅠ类基因的多态性及其进化[D]. 武汉:华中师范大学, 2012.
WANG Y Z. Polymorphism and evolution of MHC class Ⅰ genes of Rhscophorus omeimontis and Rhscophorus megacephalus[D]. Wuhan:Central China Normal University, 2012. (in Chinese)
[31] 孟祥阁. 抗阻、耐力及混合运动对C57小鼠股四头肌MHC各亚型基因表达的影响[D]. 上海:华东师范大学, 2010.
MENG X G. The mechanisms and the recovery process of exercise-induced fatigue[D]. Shanghai:East China Normal University, 2010. (in Chinese)
[32] 王丛阳, 王辉. 肌球蛋白轻链的调节及其对肿瘤细胞增殖、转移的影响[J]. 医学综述, 2010, 16(21):3251-3254.
WANG C Y, WANG H. Regulation of myosin light chain and its effect on tumor proliferation and metastas[J]. Medical Recapitulate, 2010, 16(21):3251-3254. (in Chinese)
[33] 李航, 王彬彬, 高秉仁, 等. 一个新的ACTC1基因5'端剪切位点突变可能在先天性心脏病室间隔缺损发病中起重要作用[J]. 中国组织工程研究, 2012, 16(11):2087-2090.
LI H, WANG B B, GAO B R, et al. A novel 5' splice site mutation in ACTC1 gene may play an important role in congenital ventricular septal defect[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(11):2087-2090. (in Chinese)

Mongolian Horses Transcriptome Differential Expression Analysis before and after a High Load Exercise Training

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